Animal clinical experience sharing | Simple rat respiratory anesthesia protocol

Posted by Yi Yin on

In animal experiments, rats are often used as experimental objects. When doing some injury operations such as surgery and injection, this requires anesthetizing the rats and then performing related experiments.

There are two commonly anesthesia methods: injection and inhalation. Inhalation method has little toxicity and harm to rats, and there is no injection trauma. Equiped with a respiratory anesthesia machine, it can be perfectly suitable for a series of experiments that require continuous anesthesia for several days and months.


Diethyl ether and isoflurane are commonly used for inhaled anesthetics. Such volatile anesthetics can produce short-term effects after direct inhalation in rats for a long time. However, the rat-specific respiratory anesthesia machine has a high cost, and in the case of a low need for anesthesia time in the experiment, the traditional animal respiratory anesthesia machine can be used with professional accessories to achieve corresponding results. In addition, this article will introduce a convenient anesthesia method for short-term measurement of vital signs in rats:

During anesthesia, the rats were placed in a container with a certain space, and the top was sealed with a cotton ball to ensure air flow. Then, put a cotton ball dipped in ether or isoflurane into the container. When the animal falls, the tension of the limbs is significantly reduced, and the skin pain disappears, which indicates that the animal has entered an anesthesia state, and surgery and physical signs can be performed at this time.

During the experiment, pay attention to observe the state of the rats. When the movement of limbs or beards occurs, a cotton ball dipped in ether or isoflurane can be placed on the nose of the rat to maintain anesthesia. One thing to note: During the operation or test, the experimenter should be in an upper position to prevent inhalation of volatile anesthetic gas.

"During the anesthesia process, we should pay attention to the condition of the animals and observe the reaction of the animals. Otherwise, it may easily cause the animals to be anesthetized too deeply and cause death. If there is an overdose of anesthesia, such as animals breathing very slowly and irregularly, 10% caffeine should be used when the heartbeat is very weak, 0.1% / kg intravenously or 10% colamin 0.2-0.5ml / kg intravenous or intramuscular injection. "

          - Lilac Garden Experimental Animals and Histology Technical Discussion Edition


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